亚洲男人天堂男人天堂-中文字幕在线中文字幕日亚韩一区-六月丁香婷婷久久综合-日本二区三区不卡视频

產(chǎn)品展示
您現(xiàn)在的位置:首頁 > 產(chǎn)品中心 > > 進口檢測試劑 > Prospect 難辨梭菌毒素A/B微孔板檢測試劑盒
Prospect 難辨梭菌毒素A/B微孔板檢測試劑盒

Prospect 難辨梭菌毒素A/B微孔板檢測試劑盒

型    號:
報    價:
分享到:

直接定性酶免檢測新鮮的、凍存的或Cary-Blair培養(yǎng)基保存的糞便樣本中的難辨梭菌毒素A/B

  • 產(chǎn)品描述

ProSpecT C. DIFFICILE TOXIN A/B MICROPLATE ASSAY

Code: R244596

Use
ProSpect™ C. difficile Toxin A/B Microplate Assay is a qualitative enzyme immunoassay (EIA) for the detection of C. difficile Toxin A and B in human faecal specimens from patients suspected of havingClostridium difficile disease. The test is intended for use as an aid in diagnosis of Clostridium difficile-associated disease (CDAD).

Background
Clostridium difficile, a Gram-positive, anaerobic, spore-forming bacillus, is the most common identifiable cause of antibiotic-associated diarrhoeal disease1,2. The disease occurs when treatment with broad-spectrum antibiotics suppresses bacteria in the normal intestinal flora, allowing opportunistic growth of toxigenic strains of C. difficile. The toxins produced by C. difficile, designated Toxin A and Toxin B, have potent enterotoxic and cytotoxic effects, respectively. The severity of the disease may range from uncomplicated diarrhoea to conditions  known as pseudomembranous colitis (PMC), characterised by nausea, abdominal pain, watery diarrhoea, dehydration, low grade fever and the appearance of raised yellow plaques over the colorectal mucosa. Fulminating colitis may be fatal if untreated. Nosocomial outbreaks of C. difficile  gastrointestinal illness and relapses may occur11. Antibiotic treatment directed towards C. difficile can help resolve the disease.

Diagnosis is usually performed through the detection of one or both C. difficile toxins3-12. A cell culture based cytotoxin assay is considered the reference method, but is relatively time-consuming to perform. Immunoassays detecting either Toxin A, alone, or Toxin A and B, together, have become established tools in the diagnosis of C. difficile disease4,5. The existence of non-toxigenic variants of C. difficilesupports the use of toxin based assays for definitive diagnosis. Nucleic acid probe based assays have been used experimentally, but may be complicated by the fact the organism may present asymptomatically in about 50% of infants, 20-30% of hospitalised patients and in 2-3% of healthy adults6,7. The clinical significance of detecting both Toxin A and B is not fully understood. While most disease-causing strains produce both toxins, which may act synergistically, documented cases of disease caused by Toxin B only strains of C. difficile suggest that it is clinically important to assay for both Toxin A and B8,9,10.

Description
The ProSpecT C. difficile Toxin A/B test detects the presence of Toxin A and B in clinical stool specimens through the use of specific antibodies. Microwell strips are coated with mouse monoclonal anti-Toxin A and rabbit anti Toxin B antibodies. A stool specimen  can  be diluted in Sample Diluent or used directly if pre-diluted in modified Cary-Blair medium. The sample is added to a microwell, allowing the toxins, if present, to bind to the immobilised antibodies. After washing to remove unbound components, a conjugate reagent containing goat anti-Toxin A-HRP and rabbit anti-Toxin B-HRP is added to each well. Unbound conjugate is removed by washing and a chromogenic substrate solution is added to detect the presence of bound toxin. A stop reagent is added and the test results are read visually or spectrophotometrically. The presence of a yellow colour indicates the presence of toxin.

For further information about the test and its use, including limitations, please see the IFU.

Material Supplied

Microplate (12 strips of 8 wells)Coated with mouse anti-Toxin A and Rabbit anti-Toxin B
Enzyme conjugate (25ml)Peroxide labelled goat anti-Toxin A and rabbit anti-Toxin B
Positive control (4ml)C. difficile Toxin A and Toxin B supernatant with 0.01% thimerosal
Negative control (4ml)Buffered solution with 0.1% sodium azide
Sample diluent (110ml)Buffered solution with 0.1% sodium azide
Wash buffer (110ml)10X concentrate buffered solution with 0.1% thimerosal
Colour substrate (25ml)TMB in buffer
Stop Solution (6ml)1.0N sulphuric acid (corrosive)
Disposable plastic transfer pipettes (100) 
Procedure card (1) 
Instructions for use (1) 
Plate cover (1) 

 

Materials Required but Not Supplied
(1) Stool specimen sample containers (2) Disposable test tubes (3) Timer (measuring minutes) (4) Wash bottle or dispenser for Wash Buffer (5) Distilled or deionised water.

Optional material not provided
(1) Microplate reader (spectrophotometer) capable of reading bichromatically 450/620-650nm (2) Cotton or rayon tipped applicator sticks (3)Micropipette to deliver 200μl volumes (5) Vortex mixer with plate adapter or shaker (6) Modified Cary-Blair transport medium.

Ref

  1. Bartlett, J.G., 2002 N. Engl. J. Med. 346(5):334-339.
  2. Kelly, C.P. and LaMont, L.T., 1988. Ann. Rev. Med. 49:375-390.
  3. Wilkins, T. and Lyerly, D.M., 2003. J. Clin. Microbiol. 41:531-534.
  4. O’Connor, D., Haynes, P., Cormican, M., Collins, E., Corbette-Feeney, G. and Cassidy, M., 2001. J. Clin. Microbiol. 39:2846-2849.
  5. Turgeon, D.K., Novicki, T.J., Quick, J., Carlson, L., Miller, P., Ulness, B., Cent, A., Ashley, R., Larson, A., Coyle, M., Limaye, A.P., Cookson, B.T. and Fritsche, T.R., 2003. J. Clin. Microbiol. 41:667-670.
  6. Gumerlock, P.H., Tang, Y.J., Weiss, J.B. and Silva Jr, J., 2003. J. Clin. Microbiol. 31:507-511.
  7. Belanger, S.D., Boissinot, M., Clairoux, N., Picard, F.J. and Bergeron, M.G., 2003. J. Clin. Microbiol. 41:730-734.
  8. Limaye, A.P., Turgeon, D.K., Cookson, B.T. and Fritsche, T.R., 2000. J. Clin. Microbiol. 38:1696-1697.
  9. Alfa, M.J, Kabani, A., Lyerly, D., Moncrief, S., Neville, L.M. Al-Barrak, A., Harding, G.K.H., Dyck, B. Olekson, K. and Embil, J.M., 2000. J. Clin. Microbiol. 38:2706-2714.
  10. Barbut, F., Lalande, V., Burghoffer, B., Thien, H.V., Grimprel, E. and Petit, J., 2002. J. Clin. Microbiol. 40:2079-2083.
  11. Lyerly, D.M., Neville, L.M., Evans, D.T., Fill, J., Allen, S., Greene, R., Sautter, R., Hnatuck, P., Torpey, D.J. and Schwalbe, R., 1998. J. Clin. Microbiol. 36:184-190.
  12. Nicholson, G. and Jones, M., 1999. Br. J. Biomed. Sci. 56:204-208.
廣州健侖生物科技有限公司(hnfxtuz.com) 熱門產(chǎn)品:喹諾酮類檢測試劑盒,西尼羅河檢測試劑,基孔肯雅熱試劑,寨卡檢測試劑,疫病核酸試劑
地址:廣東省廣州市番禺區(qū)石樓鎮(zhèn)清華科技園創(chuàng)啟路63號A2棟101 Email:service@jianlun.com
ICP備:粵ICP備11063766號 GoogleSitemap 技術支持:化工儀器網(wǎng) 管理登陸 返回首頁
日韩一区二区三区无吗电影| 亚洲综合偷拍日韩av| 婷婷精品国产欧美精品亚洲| 亚洲精品丝袜成人偷拍| 久久国产精品99亚洲| 91在线国产手机视频| 一区不卡在线视频免费国产| 人妻一区二区三区免看| 五十路六十路美熟人妻| 亚洲国产成人午夜精品| 日本少妇人妻中文字幕| 国产欧美一区二区另类精品 | 岛国大片日韩在线观看| 日本人妻风俗店中文字幕| 亚洲福利一区二区在线| 亚洲综合av伊人久久| 亚洲日本韩国三级一区| 亚洲综合一区二区三区四区在线 | 午夜视频在线观看免费版| 亚洲精品国产自在现线| 国产精品1区2区久久久| 国产精品久久护士96| 在线成人免费日韩视频| 日本顶级片一区二区三区| 国产精品乱码精品久久久| 开心激情欧美一区二区| 国产区一区二区三在线播放| 好看的中文字幕人妻少妇| 人妻一本久道久久综合鬼色| 日韩精品一区二区三区中文| 午夜免费视频观看在线| 69精品一区二区蜜桃视频| 久久精品一区二区婷婷| 久久国产精品av在线观看| 欧美 日韩亚洲一区| 亚洲欧美av中文日韩二区| 天堂中文在线免费观看av| 一区不卡在线视频免费国产| 亚洲成人精品夫妻av| 欧美口爆吞精在线观看| 日韩黄色一级片免费看|